GENERAL INFORMATION
A*CGT Pre-Symposium Workshop:
Advances in Bioprocessing Capabilities
16 May 2023
1:00 to 5:15 pm
Seminar Room 03-46, Institute of Molecular and Cell Biology, Biopolis
Advances in Bioprocessing Capabilities
16 May 2023
1:00 to 5:15 pm
Seminar Room 03-46, Institute of Molecular and Cell Biology, Biopolis
We are pleased to welcome you to attend the A*STAR Advances in Cell and Gene Therapy (A*CGT) Pre-Symposium Workshop on 16 May 2023, which intends to expose early career researchers to new techniques, workflows, and instruments applicable in the cell therapy and bioprocessing space through hands-on interaction. The half-day workshop will feature speakers from the industry, followed by a networking session and a hands-on session (three demos over 30 min each).
The pre-symposium is jointly organized by A*STAR Research Entities (Institute of Molecular and Cell Biology (IMCB), Singapore Institute of Manufacturing Technology (SIMTech), Bioprocessing Technology Institute (BTI) and Singapore Immunology Network (SIgN)), Singapore Cell Therapy Advanced Manufacturing Programme (STAMP) and Research Officer Career Kinetics (ROCK).
Please register and join us to learn the latest developments and interact with our speakers. As the workshop is open to 36 attendees only, registration is on a first come, first served basis. We look forward to your participation in the workshop.
The pre-symposium is jointly organized by A*STAR Research Entities (Institute of Molecular and Cell Biology (IMCB), Singapore Institute of Manufacturing Technology (SIMTech), Bioprocessing Technology Institute (BTI) and Singapore Immunology Network (SIgN)), Singapore Cell Therapy Advanced Manufacturing Programme (STAMP) and Research Officer Career Kinetics (ROCK).
Please register and join us to learn the latest developments and interact with our speakers. As the workshop is open to 36 attendees only, registration is on a first come, first served basis. We look forward to your participation in the workshop.
ABSTRACTS
PhenomeX
Instrument to showcase: Lightning® Optofluidic System
Talk title: Comprehensive Single T cell profiling on the PhenomeX Beacon® and Lightning® platforms
Talk abstract: Polyfunctional T cells, which produce multiple cytokines, are considered to be the most potent anti-tumour effector T cells. To find polyfunctional T cells in a heterogeneous sample, scientists must be able to characterize cytokine secretion and cytotoxicity at the single-cell level. A variety of techniques are currently used to assess T cell function, including flow cytometry, live-cell imaging, and chromium release to assess target cell killing; and ELISA/ELISPOT to measure cytokine expression. However, these traditional analytical methods are complex, labour/cost intensive, and have high sample requirements, thus generating deep characterization datasets to define critical quality attributes has not been possible. In addition, as these techniques are necessarily applied to separate cell populations, any relationships can only be inferred, and multiple functional events can never be directly observed on the same cell. During this seminar, we will describe how the newly released PhenomeX Opto® T Cell Profiling workflow on the Beacon® and Lightning® systems makes it possible to comprehensively profile single T cells and link this phenotypic and functional data to the genotype of those same cells. Individual T cell-target cell interactions are precisely assembled in thousands of nanolitre-sized NanoPen® (pen) chambers across an OptoSelect®️ microfluidic chip using light in a process called Optoelectropositioning (OEP™). T cells can be selectively penned based on fluorescent cell surface markers and co-cultured with target cells and cytokine capture beads. Time lapse brightfield and fluorescence imaging is then used to assess T cell function (cytokine secretion) and killing activity. Importantly, individual T cells of interest are exported for downstream analysis such as sequencing.
Miltenyi Biotec
Instrument to showcase: CliniMACS® Prodigy
Talk Title: CGT workflow on the CliniMACS® Prodigy
Talk Abstract: Miltenyi Biotec is a leader in the clinical research field with strong emphasis on immunotherapy, tissue regeneration, and graft engineering. For more than 20 years, the automated CliniMACS® Cell Separation System has enabled consistent generation of cellular products for a wide range of clinical applications by leveraging the renowned MACS® Technology, a proven magnetic separation methodology. In combination with the high-quality MACS GMP and CryoMACS® product lines as part of the CGT workflow, GMP-compliant cell processing and manufacturing on the CliniMACS® Prodigy System has generated more than 50,000 cellular products for therapy procedures. The versatility and capabilities of the CliniMACS® Prodigy are extensive and to appreciate them holistically, we will introduce the novel design and validated processes of the CliniMACS® Prodigy.
Thermo Fisher Scientific
Instrument: CTS™ DynaCellect™ Magnetic Separation System
Talk Title: Introducing the CTS™ DynaCellect™ Magnetic Separation System
Thermo Fisher Scientific has a line of Gibco™ CTS™ closed cell processing system developed to function as either a stand-alone system or integrated into a workflow that can help with standardization and quality maintenance during manufacturing. As a part of this workflow, the Gibco™ CTS™ DynaCellect™ Magnetic Separation System has been designed for column-free and scalable cell processing with the GMP-grade CTS™ Dynabeads™ platform. The instrument utilizes an integrated magnet-rocker and a fluidics panel for targeted cell separation such as T cell isolation and NK enrichment.
Using Gibco™ CTS™ DynaCellect™ with CTS CD3/CD28 Dynabeads allows both isolation and activation in a single-step. We consistently achieve >85% isolation efficiency of target cells with >95% purity with no effect on cell viability. Furthermore, automated bead removal using the Gibco™ CTS™ DynaCellect Bead Removal Kit resulted in >85% target cell recovery. Overall, the DynaCellect is highly scalable allowing continuous flow over the rocker-magnet to ensure rapid processing of volumes suitable for autologous and allogenic therapies.
Instrument to showcase: Lightning® Optofluidic System
Talk title: Comprehensive Single T cell profiling on the PhenomeX Beacon® and Lightning® platforms
Talk abstract: Polyfunctional T cells, which produce multiple cytokines, are considered to be the most potent anti-tumour effector T cells. To find polyfunctional T cells in a heterogeneous sample, scientists must be able to characterize cytokine secretion and cytotoxicity at the single-cell level. A variety of techniques are currently used to assess T cell function, including flow cytometry, live-cell imaging, and chromium release to assess target cell killing; and ELISA/ELISPOT to measure cytokine expression. However, these traditional analytical methods are complex, labour/cost intensive, and have high sample requirements, thus generating deep characterization datasets to define critical quality attributes has not been possible. In addition, as these techniques are necessarily applied to separate cell populations, any relationships can only be inferred, and multiple functional events can never be directly observed on the same cell. During this seminar, we will describe how the newly released PhenomeX Opto® T Cell Profiling workflow on the Beacon® and Lightning® systems makes it possible to comprehensively profile single T cells and link this phenotypic and functional data to the genotype of those same cells. Individual T cell-target cell interactions are precisely assembled in thousands of nanolitre-sized NanoPen® (pen) chambers across an OptoSelect®️ microfluidic chip using light in a process called Optoelectropositioning (OEP™). T cells can be selectively penned based on fluorescent cell surface markers and co-cultured with target cells and cytokine capture beads. Time lapse brightfield and fluorescence imaging is then used to assess T cell function (cytokine secretion) and killing activity. Importantly, individual T cells of interest are exported for downstream analysis such as sequencing.
Miltenyi Biotec
Instrument to showcase: CliniMACS® Prodigy
Talk Title: CGT workflow on the CliniMACS® Prodigy
Talk Abstract: Miltenyi Biotec is a leader in the clinical research field with strong emphasis on immunotherapy, tissue regeneration, and graft engineering. For more than 20 years, the automated CliniMACS® Cell Separation System has enabled consistent generation of cellular products for a wide range of clinical applications by leveraging the renowned MACS® Technology, a proven magnetic separation methodology. In combination with the high-quality MACS GMP and CryoMACS® product lines as part of the CGT workflow, GMP-compliant cell processing and manufacturing on the CliniMACS® Prodigy System has generated more than 50,000 cellular products for therapy procedures. The versatility and capabilities of the CliniMACS® Prodigy are extensive and to appreciate them holistically, we will introduce the novel design and validated processes of the CliniMACS® Prodigy.
Thermo Fisher Scientific
Instrument: CTS™ DynaCellect™ Magnetic Separation System
Talk Title: Introducing the CTS™ DynaCellect™ Magnetic Separation System
Thermo Fisher Scientific has a line of Gibco™ CTS™ closed cell processing system developed to function as either a stand-alone system or integrated into a workflow that can help with standardization and quality maintenance during manufacturing. As a part of this workflow, the Gibco™ CTS™ DynaCellect™ Magnetic Separation System has been designed for column-free and scalable cell processing with the GMP-grade CTS™ Dynabeads™ platform. The instrument utilizes an integrated magnet-rocker and a fluidics panel for targeted cell separation such as T cell isolation and NK enrichment.
Using Gibco™ CTS™ DynaCellect™ with CTS CD3/CD28 Dynabeads allows both isolation and activation in a single-step. We consistently achieve >85% isolation efficiency of target cells with >95% purity with no effect on cell viability. Furthermore, automated bead removal using the Gibco™ CTS™ DynaCellect Bead Removal Kit resulted in >85% target cell recovery. Overall, the DynaCellect is highly scalable allowing continuous flow over the rocker-magnet to ensure rapid processing of volumes suitable for autologous and allogenic therapies.
ENQURIES
For enquiries, please contact us at: ACGTsymposium@imcb.a-star.edu.sg
ORGANISED BY
|
|
|